Journal article

Influence of chlorophyll concentrations on the reliability of measurements with an oxygen electrode when studying photosynthetic efficiency in bioassays using microalgae

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Author list: Danilov, Roman;Ekelund, Nils

Publication year: 2000

Start page: 63

End page: 67

Number of pages: 5

ISSN: 0921-8971

DOI: http://dx.doi.org/10.1023/A:1008189230953

View additional information: View in Web of Science


Abstract

Euglena gracilis and Chlamydomonas reinhardtii were used as model organisms to establish the best conditions for studying photosynthetic efficiency using the Light Pipetteexperimental system, which enables sensitive detection of changes in oxygen evolution/consumption and an easy collection and digitalisation of data. Chlorophyll concentrations of 0.005, 0.025, 0.050 and 0.075 mg mL-1 were investigated using different light regimes. Cultures of E. gracilis at the same chlorophyll concentration absorbed more light (measured at 580 µmol m-2 s-1) than those of C. reinhardtii. Cell density had a considerable effect on the reliability of measurements. Chlorophyll concentrations between 0.025 mg(Chl) ml-1 and 0.050 mg(Chl) ml-1 can be recommended when applying the Light Pipette system in bioassays using microalgae.


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