Conference proceedings article

A comparative study including Clamydomonas reinhardtii and Chlamydomonas raudensis Ettl. strains SAG 49.72 and UWO 241 focusing on phototaxis.

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Author list: Ekelund, Nils;Falk, Stefan;Pocock, Tessa

Publication year: 2005


Abstract

Phototaxis is movement induced by light; this phenomenon has been detected in several solitary green algae species and generally works as a balancing force against gravitaxis, creating a system for optimum vertical placing in relation to irradiance, (energy -demands/restrictions). We study phototaxis in the mesophilic green algae, Chlamydomonas reinhardtii and C. raudensis SAG 49.72, relative to phototaxis in the from Antarctica newly isolated obligate psychrophilic strain of C. Raudensis (UWO 241) (henceforth called UWO 241). This species has some unusual movement patterns (Pocock et. al 2004) which function is yet to be revealed. Phototactic movement has not earlier been monitored in the normal conditions (high salinity, low temperature) for this strain, though when exposed to extreme temperatures (25?C) the organism displays movement patterns interpreted as positive phototaxis. (Pocock et. al 2004). This behaviour should lead to a more rapid destruction of the organism due to the damaging high light (high energy input). The study shows that the three species responds different to light stimuli, when temperature is set to 12? C (culturing temperature) or the respective optimum culture temperatures. Light response curves of phototaxis in the range of 0,5 to 3000 µmol quanta m-2 s-1, shows that C. raudensis SAG 49.72 produce positive phototaxis over the entire range while C. reinhardtii mainly shows negative reactions. UWO 241 seem to be unaffected by irradiance, thus showing only nondirectional behaviour. In a second set of experiments the cells were kept in Petri dishes and exposed to 3000 µmol quanta m-2 s-1 under 40 min, in a temperature range of 5?C to 25?C for each sample. The results show that C. reinhardtii was negatively phototactic at 5?C, switching to positive phototaxis at 25?C.


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